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Information × Registration Number 0221U102703, 0119U100307 , R & D reports Title Molecular and cellular mechanisms of TRP canalopathy. It is the same alloy with NKM composition. popup.stage_title Head Zholos Oleksandr V., Доктор біологічних наук Registration Date 09-02-2021 Organization Taras Shevchenko National University of Kyiv popup.description2 Object of study: expression, mechanisms of activation and functions of receptor-directed TRPC4 channels, mechano-, osmo- and heat-sensitive TRPV4 channels and TRPM8 cold and menthol receptors in smooth muscle, skeletal muscle and epithelial cells in normal and under conditions of pathologies of different genes. Subject of research: interrelation of key links of TRP-mediated signaling in interaction with other potential-dependent channels, in particular Ca2 + -activated K + channels of high conductivity (maxiK, BKCa) and potential-directed Ca2 + channels of L-type (Ca-L) in regulation of vascular tone and contractile secretory function of the intestine and maintenance of the barrier function of the epithelium and endothelium. Objective: to determine the expression and function of sensory and receptor-directed TRP channels, as well as their interaction with other potential-dependent channels (including BKCa, Ca-L), as potential target molecules for the correction of canalopathies in hypertension of various genesis, early nonmotor manifestations in Parkinson's disease and disorders barrier function of the epithelium and endothelium. Research methods: electrophysiological (patch clamp), calciummetry using calcium-sensitive fluorescent dye Fura-2, in vitro strain gauge, Ussing chamber for registration of transepithelial tissue resistance and transport of ions through the intestinal mucosa, permeability assessment for FITCde etc. (specified in the Terms of Reference). A comparative quantitative analysis of the activation and inactivation properties of potential-dependent L-type Ca channels was performed. The biophysical characteristics of stationary input "window currents" suggest their important role in generating tonic contraction of arterial smooth muscle and, accordingly, hypertensive states of various genesis. Product Description popup.authors Horbach Oleksandr I. Dovbynchuk Taisa V. Dryn Darіya O. Melʹnyk Mariya I. Nozdrenko Dmytro М. Svyetlova Nataliya B. Soroka Vasyl M. Stetskaya Victoriya O. popup.nrat_date 2021-02-09 Close