Registration Number
0221U103085,
0119U100168
, R & D reports
Title
Biochemical aspects of the functioning of metabolic and signaling pathways under the influence of protein molecules in the conditions of normal and pathologically altered metabolism.
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Head
Savchuk Olexiy M., Доктор біологічних наук
Registration Date
14-02-2021
Organization
Taras Shevchenko National University of Kyiv
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Object of research: system of proteolysis in conditions of bladder cancer, chronic pancreatitis; peptide pools from tissues of objects with pathologically altered metabolism
Objective: to investigate the state of the proteolysis system in patients with bladder cancer and animals with a model of chronic pancreatitis, as well as to identify, characterize peptide pools from tissues of objects with pathologically altered metabolism (bladder cancer, chronic pancreatitis separately and on the background of diabetes mellitus 1 type, atherothrombotic and cardioembolic ischemic stroke) and to investigate the effect of peptide pools on the activity of some factors of the hemostasis system.
Research methods: chromatographic, electrophoretic, enzyme-linked immunosorbent assay, spectrophotometric and methods of mathematical statistics.
For the first time, the fraction of trypsin-like enzymes from blood plasma in patients with bladder cancer was obtained and characterized and fundamental differences in its composition were revealed, namely the absence of stable covalent complexes of proenzymes with effector molecules and the appearance of elastase fragments. A violation of proteolytic balance with the development of bladder cancer, the manifestation of which is an increase in total proteolytic activity, the activity of matrix metalloproteinases and serine proteinases. These changes occur against the background of changes in the content and activity of key inhibitors in plasma, namely increased activity of α2-antiplasmin, α2-macroglobulin in plasma of patients with PCM at different stages and decreased activity of α1-antitrypsin in patients with stage III bladder cancer.
Product Description
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Bazmanova Lyudmyla М.
Galenova Tetyana I.
Dunich Alina A.
Konotop Yevheniia O.
Olefirenko Viktoriya V.
Raksha Nataliya G.
Snigur Galyna O.
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2021-02-14
