Registration Number
0221U103670,
0118U000137
, R & D reports
Title
5S ribosomal DNA: molecular organization and evolution in changing environmental conditions.
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Head
Volkov Roman A., Доктор біологічних наук
Registration Date
24-02-2021
Organization
Yuriy Fedkovych Chernivtsi National University
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Research report: 168 pages, 132 ref., 44 figs., 29 tables.
GENETIC POLYMORPHISM, 5S RIBOSOMAL DNA, MOLECULAR MARKERS, PHYLOGEOGRAPHY, ABIOTIC STRESS.
The object of study is the organization and molecular evolution of repetitive sequences in the genomes of eukaryotic organisms.
The aim of the study was to investigate the molecular organization and polymorphism of 5S rDNA of angiosperms in connection with adaptation to changes in environmental conditions and to identify the factors that determine the variability of rDNA.
Research methods: molecular genetic (PCR, cloning of DNA fragments, sequencing), biochemical (determination of enzyme activity and concentration of metabolites), bioinformatics (DNA sequence analysis, construction of philodendrograms, database search).
5S rDNA of 15 populations of the Antarctic plant Deschampsia antarctica, 9 samples of other species of Deschampsia and 15 samples of related genera of the Poeae tribe were cloned and sequenced. The genetic relatedness of the studied species and the patterns of 5S rDNA variability were assessed. In the genus Deschampsia, 3 structural classes of 5S rDNA have been identified, which differ significantly in the structure of the intergenic spacer (IGS). 5S rDNA was sequenced for 110 samples of Acer, Aesculus, Quercus, Castanea and Gentiana species from 20 European (including different regions of Ukraine) and Asian countries. Widespread SNP mutations that can be used for genotyping have been identified. During the ice age, species of the genera Quercus and Acer have been found to have survived in several refugia in the Mediterranean and the Caucasus. In the postglacial period, plants migrated to Central Europe in several ways. At the molecular level, species of the genus Quercus have evolved more slowly than species of the genus Acer and have undergone little differentiation into local races.
Product Description
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Ivanovych Yaroslav I.
Ishchenko Olga O.
Derevenko Tatyana O.
Kasiyanchuk Ruslana M.
Melnyk Vitaliy M.
Panchuk Irina I.
Tynkevich Yurij O.
Cherevatov Alexandr V.
Shelyfist Antonina Ye.
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2021-02-24
