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Information × Registration Number 0221U104465, 0119U103905 , R & D reports Title Role of mitochondrial protein MRPS18-2 and RB in cell stemness control and carcinogenesis, 2.2.5.428 popup.stage_title Head Kashuba Elena V., Доктор біологічних наук Registration Date 25-03-2021 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2  First, proteins encoded by genes of the MRPS18 family (1-3), were translated in vitro, using constructs in the pGEM vector. FLAG-tagged constructs for genes of MRPS18 family (1-3) were created as well. Conjugation of ubiquitin was performed in vitro, using reticulocyte lysates. It was found that all proteins of the MRPS18 family have different half-lives, the most unstable is the MRPS18-3 protein, which degrades in a few minutes. The MRPS18-2 protein is practically not degraded. The half-life of MRPS18-1 protein is 90 minutes. To investigate the possibility of ubiquitination of MRPS18 family proteins in vivo, tumor cell of MCF7 and Cos7 lines were transfected with FLAG-tagged constructs, together with the HA-Ub plasmid. Using double staining and fluorescence microscopy, co-localization of MRPS18-1 and -2 proteins with ubiquitin was observed in transfected cells, in contrast to MRPS18-3. Product Description popup.authors Vovkolup Kateryna V. Kalman Sergii S. Kashuba Elena V. Kovalevska Larysa M. popup.nrat_date 2021-03-25 Close