Registration Number
0221U104848,
0120U102186
, R & D reports
Title
Recording kinetics of a thin dynamic holographic grating (Raman-Nath) for the study of nano-bio materials based on retinal-protein biological photoreceptors with low intensity of absorption saturation
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Head
Korchemska Olena Ya., Кандидат фізико-математичних наук
Registration Date
25-04-2021
Organization
International center "Institute of applied optics" National academy of science
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It is proposed to study light-induced changes in refractive index and lifetime of photocycle intermediates in bacteriorhodopsin (BR) samples using kinetics of a thin dynamic holographic grating (Raman-Nat), as there is data in the hybrid nanosystem BR a significant increase in the nonlinear refractive index compared to the BR of the solution. Until now, only the method of absorption spectroscopy with time separation was used for this purpose. But it is well known that the most sensitive to light-induced changes in refractive index is holography, and BR and its genetic mutants are extremely promising for dynamic holographic recording. The thin dynamic holographic grating (Raman-Nat) allows to register the kinetics of the first orders of the diffracted beam in the mode of self-diffraction of recording beams, which significantly increases the value of the intensity of the diffracted beam and simplifies measurements. Based on the changes under the action of light of the complex refractive index for BR molecules combined into trimers, taking into account the supramolecular interaction between photoactivated BR molecules of the trimmer, it is proposed the model of thin dynamic holographic grating of intensities (Raman-Nat) in the mode of self-diffraction of recording beams.
Product Description
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Savchuk Alla V.
Stepanchikov Dmitrij A.
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2021-04-25
