Registration Number
0221U106019,
0120U104519
, R & D reports
Title
Study on the structural features of human translation elongation complex eEF1B
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Head
Negrutskyii Borys S., Доктор біологічних наук
Registration Date
23-09-2021
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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Research object – Spatial organization of translation elongation factors eEF1Bβ і eEF1Bγ.
Purpose of work – To reveal for the first time the spatial organization of human translation elongation factors eEF1Bβ і eEF1Bγ.
Research methods – molecular biological (PCR, cloning, mutagenesis, expression of recombinant proteins), physico-chemical (HDX-MS, analytical centrifugation,V-ray scattering), biochemical (chromatographic purification of individual proteins, Western blot), bioinformatics (molecular modeling).
Results and novelty:
For the first time the structural dynamics of eEF1Bβ and eEF1Bγ proteins was elucidated experimentally.
For the first time molecular form of multimeric eEF1Bβ protein was determined and the level of its multimerization was examined.
For the first time the computer models of spatial organization of eEF1Bβ and eEF1Bγ were created.
Product Description
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Bondarchuk Tetyana V.
Gurianov Dmytro S.
Novosylna Oleksandra V.
Tsuvariev Oleksandr Yu.
Shalak Viacheslav F.
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2021-09-23
