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Information × Registration Number 0221U106059, 0121U113126 , R & D reports Title Differential control of regulatory networks involved in the maintaining of epithelial-mesenchymal plasticity of breast adenocarcinoma cells popup.stage_title Head Filonenko Valerii V., д.б.н. Registration Date 04-10-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  Breast cancer is one of the most common oncological disease among women in the world and in Ukraine. Antitumor therapy is based on the suppression of factors detected in overexpressed or overactivated form compared to normal tissue. Often, the combined suppression of several targets increases the effectiveness of anticancer therapy. One of the overactivated and overexpressed signaling cascades in breast carcinomas is the S6K signaling pathway. In a number of clinical studies, the signaling pathway and S6K proper act as targets for anticancer therapy. This is due to their involvement in the regulation of cell proliferation and migration on the one hand, and the ability of ligands to independently activate the estrogen receptor kinase by ribosomal protein S6 on the other. However, due to the existence of a number of S6K isoforms, there is no understanding of the differences in their activity. The expression spectrum of genes involved in the progression of breast tumors in MCF-7 cell lines with differential expression of S6K1 isoforms generated by genomic editing using CRISPR technology was investigated. The qPCR method was used to study and detect changes in the levels of gene expression of molecules involved in adhesion (Tjp1, Icam1, Itgb1, Cfl1), control of inflammation and immune response (Il1a, Tnf, Il6, Ccl2, Cxcl5, Lcn2), cell reprogramming, Poux2, Myc , Nanog), proteinases that degrade the extracellular matrix (Mmp2, Mmp9, Mmp13, Mmp14, Ctsd1), genes of components of the plasminogen activation system (uPA / uPAR / PAI-1) involved in the control of migration and invasion, and components of the signaling system vitamin D3 (VDR, CYP27B1), involved in the control of differentiation in the studied sublines of adenocarcinoma cells MCF7 depending on the level of expression of isoforms of kinase S61. The largest changes were found in MCF7 cells expressing p60 isoform S6K1. Product Description popup.authors Bdzola Anna Volodymyrivna Garifulin Oleg Minirovytch Gotsulyak Nazar Yaroslavovytch Khoruzhenko Antonina Ivanivna popup.nrat_date 2021-10-04 Close