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Information × Registration Number 0221U106713, 0120U104115 , R & D reports Title Development of new nanozymes as catalytic elements for enzymatic kits and chemo/biosensors popup.stage_title Head Gonchar Mychailo V., Доктор біологічних наук Registration Date 17-12-2021 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description2  For the first time: - different cytotoxic effect of synthesized nanozymes (NZs) on the yeast Ogataea polymorpha cells was shown; - uptake of NZs into the yeast cells was detected, which leads to an increase in the enzymatic activity of the cells; - for the first time in the world, the possibility of replacing the natural enzyme catalase with artificial "catazyme", which restores the ability of non-catalase yeast cells to grow on methanol, has been shown; - molecular-genetic analysis and sequencing of the genome locus of the yeast strain Ogataea polymorpha C-105 revealed two mutations in the catalase gene: replacement of C at the 895 position of the coding sequence by T and deletion of C at the 896 position; this combination leads to a shift in the reading frame and the formation of the stop codon TGA, which causes the production of non-functional, incomplete catalase protein in the cells of the strain C-105; - using artificial nanomimetics of peroxidase and natural glucose oxidase, new enzymo-nanozymetic methods for glucose assay have been developed. It has been improved: - methodology of structural characterization of NZs by physical methods: scanning electron microscopy, atomic force microscopy and positron annihilation spectroscopy; - methodology for studying the biological and cytotoxic effects of synthesized NZs on the survival of eukaryotic cells on the model of the non-conventional yeast using fluorescent dyes; - has been approved the uptake of NZs by the yeast O. polymorpha and intracellular localization of NZs, which leads to an increase in catalase, laccase, pseudo-reductase and peroxidase activitis of the cells, enriched with the corresponding NZ, when compared with control cells; - has been developed the procedure of analysis of human blood serum and fruit juices for glucose content by enzymo-nanozymatic method. Product Description popup.authors Demkiv Olha M. Stasiuk Nataliia Ye. popup.nrat_date 2021-12-17 Close