Registration Number
0221U106713,
0120U104115
, R & D reports
Title
Development of new nanozymes as catalytic elements for enzymatic kits and chemo/biosensors
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Head
Gonchar Mychailo V., Доктор біологічних наук
Registration Date
17-12-2021
Organization
Institute of Cell Biology of the National Academy of Sciences of Ukraine
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For the first time: - different cytotoxic effect of synthesized nanozymes (NZs) on the yeast Ogataea polymorpha cells was shown;
- uptake of NZs into the yeast cells was detected, which leads to an increase in the enzymatic activity of the cells;
- for the first time in the world, the possibility of replacing the natural enzyme catalase with artificial "catazyme", which restores the ability of non-catalase yeast cells to grow on methanol, has been shown;
- molecular-genetic analysis and sequencing of the genome locus of the yeast strain Ogataea polymorpha C-105 revealed two mutations in the catalase gene: replacement of C at the 895 position of the coding sequence by T and deletion of C at the 896 position; this combination leads to a shift in the reading frame and the formation of the stop codon TGA, which causes the production of non-functional, incomplete catalase protein in the cells of the strain C-105;
- using artificial nanomimetics of peroxidase and natural glucose oxidase, new enzymo-nanozymetic methods for glucose assay have been developed.
It has been improved:
- methodology of structural characterization of NZs by physical methods: scanning electron microscopy, atomic force microscopy and positron annihilation spectroscopy;
- methodology for studying the biological and cytotoxic effects of synthesized NZs on the survival of eukaryotic cells on the model of the non-conventional yeast using fluorescent dyes;
- has been approved the uptake of NZs by the yeast O. polymorpha and intracellular localization of NZs, which leads to an increase in catalase, laccase, pseudo-reductase and peroxidase activitis of the cells, enriched with the corresponding NZ, when compared with control cells;
- has been developed the procedure of analysis of human blood serum and fruit juices for glucose content by enzymo-nanozymatic method.
Product Description
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Demkiv Olha M.
Stasiuk Nataliia Ye.
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2021-12-18
