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Information × Registration Number 0221U106794, 0120U102860 , R & D reports Title Enzymatic pool of macromycetes in bioconversion of lignocellulosic residues popup.stage_title Head Radchenko Volodymyr G., Доктор біологічних наук Registration Date 20-12-2021 Organization Institute for evolutionary ecology of the National Academy Sciences of Ukraine" Ukraine popup.description2 We found that the citrate buffer system stabilizes and activates the cellulase complex of Irpex lacteus Il-11. The high activity of the cellulase complex (300U / g) allows maintaining the linear proportionality of product growth from the concentration of enzymes. The process of enzymatic saccharification of fibre should be carried out for up to 96 hours, depending on technological needs. Pre-mercerization of the paper does not have a significant advantage in the process of its saccharification, and this will reduce the process and reduce the cost of the final product. The rate of the catalytic reaction is equal to half the limiting concentration of the enzyme of approximately 20 U/g. The Michaelis-Menten constant is 0.063 M, and the maximum reaction rate is 1.9 μM/min. For the I. lacteus cellulase complex, we recommended using a substrate at a concentration of 5–7% for the highest yield of reducing sugars. The final low-temperature treatment allows to release of cellulase components and increase protein yield by 16%.  Product Description popup.authors Boiko Serhii Mykhailovych Netsvetov Maksym Viktorovych Prokopuk Yulia Serhiivna popup.nrat_date 2021-12-20 Close