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Information × Registration Number 0221U106974, 0121U109072 , R & D reports Title Development and testing of a diagnostic set for the detection of clinically significant mutations in BRAF and other tumor-associated genes in human cancer samples popup.stage_title Head Kashuba Volodymyr I., Registration Date 23-12-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  As a result of the project, the design, selection and optimization of a number of mutations in the BRAF gene at position 600 (V600E, V600E complex, V600D, V600K) (rs113488022, rs121913377, rs121913227) were performed. Positive controls in the form of plasmids with both normal and mutated sequence were synthesized. The efficiency and specificity of primers for the studied mutations were determined. The most sensitive primers to mutation A (V600E), which is the most common and clinically significant. The specificity of their work showed that they are able to detect two mutations A and C, which can be differentiated using both pairs of primers (primers A and C). Tissue and ovarian tumors of both fresh-frozen and paraffin blocks were screened. As a result, positive for A mutation samples were detected. The results were confirmed by Senger sequencing and next-generation sequencing. A protocol was developed, laboratory samples were made, and a diagnostic kit was tested to detect clinically significant mutations in the BRAF gene at the position 600. Product Description popup.authors Gerashchenko Ganna V. Gordiyuk Vasyl Vasylovych Hryshchenko Nataliya V. Kashuba Volodymyr I. popup.nrat_date 2021-12-23 Close