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Information × Registration Number 0221U107232, 0118U005478 , R & D reports Title To determine the prognostic significance of intercellular interaction mediators and differentiation clusters of substrate cells for stratification and optimization of the myelodysplastic/myeloproliferative neoplasms treatment popup.stage_title Head Tretiak Naliia M, Registration Date 29-12-2021 Organization State Institution "Institute of Hematology and Transfusiology NAMS Ukraine" popup.description2 The object 156 patients with myelodysplastic/myeloproliferative neoplasms (MDS/MPN) aged 40-79 years.The aim is to improve the criteria for prognosis of MDS progression and effectiveness of therapy based on the study of clinical and hematological, immunological data, cytogenetic parameters, assessment of changes in serum cytokine levels, the results of bone marrow cell proliferative capacity in patients with MDS/MPN.The analysis of clinical and laboratory data and results of immunological, enzyme-linked immunosorbent, cytogenetic and cultural studies of 156 patients with MDS in the initial period and after therapy, determined the immunophenotypic profile of bone marrow tumor cells by flow cytometry.The levels of cytokines TNF,VEGF and IL-I in the serum of patients with MDS RAEB I and RAEB II, their relationship with the course of the disease and response to treatment were determined.Factors that can potentially be used to assess response to treatment and predict resistance to therapy have been shown to be indicators of expression of differentiation clusters(CD34+/CD117+)on cells of myeloid origin and the level of cytokines studied. It is shown that the percentage of cells with coexpression of markers of differentiationCD34 +/CD117+≤ 11.51.5in bone marrow and the concentration of TNF in serum≤(8.300.40)pg /ml is the optimal level for predicting a complete response for lenalidomide therapy in patients with MDS RANB I.Increase in VEGF content in serum in MDS RAEB I ≥(83, 4 5.3)pg/ml predicts transformation into AML.It is shown that the proliferative potential less than 0.4 in MDS RAEB I and less than 0.1 in MDS RAEB II predicts an unfavorable course of the disease.A favorable prognosis is stated at a proliferative potential of 2.1 at MDS RAEB I and 1.6 at MDS RAEB II.The conducted researches will allow to reasonably improve the algorithm of stratification of patients according to groups of risk of progression of MDS and also to choose the most optimum therapeutic Product Description popup.authors Aladieva Olena M Andreieva Svitlana Vasylivna Bilko Ndiia Mykhailivna Basova Olha V Kubarova Valentyna O Kuiavovych Bohdana M Starodub Halyna S popup.nrat_date 2022-03-09 Close