Registration Number
0221U107270,
0121U100472
, R & D reports
Title
Development of intraoperative diagnosis method for malignant tumors determining using fluorophore-conjugated antibodies to carcinoembryonic antigens
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Head
Lyndin Mykola S., Кандидат медичних наук
Registration Date
30-12-2021
Organization
Sumy State University
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The object of research is malignant tumors of the main localizations. The subject of research is the immunophenotype of malignant tumor cells, molecular features of the interaction of fluorophore-conjugated antibodies with cancer cells in vitro and in vivo, the effectiveness of fluorescence-guided surgery in the visualization of malignant neoplasms. The aim of the work is to create a technique of intraoperative photodynamic imaging in the near infrared region of the spectrum of malignant tumors of the main localizations using fluorophore-conjugated antibodies to molecules of cancer-embryonic antigen. To achieve this goal, the following tasks were identified: to conduct immunohistochemical study of normal and tumor tissues for the expression of different types of CEACAM; perform "mapping" of the immunological profile of carcinoma; conjugate anti-CEACAM antibodies with fluorophores. Research methods - histological (hematoxylin and eosin staining) and immunofluorescent (determination of CEACAM expression) study of postoperative material; creation of fluorophore-conjugated anti-CEACAM antibodies according to the protocols of the fluorophore manufacturer; mathematical methods. The research is aimed at establishing the immunophenotype of normal and tumor tissue, determining the influence of cell adhesion molecules on morphological and molecular genetic features of carcinoma
Product Description
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Berest Oleg B.
Bova Yana A.
Varava Yuliya O.
Hyriavenko Nataliia
Zolotareva Vira H.
Karpenko Bohdana M
Kravchenko Yaroslav O
Kuzenko Yevhen V
Lyndin Mykola S.
Lyndina Yuliia M.
Oleshko Oleksandr M.
Oleshko Tetiana B
Sikora Vladyslav V
Sikora Kateryna O.
Sikora Yana V.
Soroka Yuliya A.
Tymakova Olena O.
Chyzhma Ruslana A.
Yanko Illya K.
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2022-03-09
