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Information × Registration Number 0222U000669, 0120U100749 , R & D reports Title To study the significance of alternative splicing of the SorL1 gene for impaired expression of lipoprotein lipase in patients with chronic lymphocytic leukemia affected due to Chornobyl NPP accident popup.stage_title Head Diahil Iryna S., Chumak Anatolii A., Доктор медичних наук Registration Date 14-01-2022 Organization State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine" popup.description2  Object of study: 61 chronic lymphocytic leukemia (CLL) patients, including 34 patients exposed to ionizing radiation (IR) due to the Chornobyl NPP accident (the main group), and 27 patients without exposure to IR (the group of comparison). The aim of study: to establish the prognostic value of the expression of the SORL1 gene in chronic lymphocytic leukemia patients suffered due to the Chornobyl NPP accident. Methods: clinical, molecular genetic - polymerase chain reaction (PCR), sequencing of the obtained reaction products, statistical. Equipment: thermal cycler Bio-Rad C1000 (N10450137), dispensers. The relative expression level of the main transcript of the SORL1 gene was low (1.71 ± 0.55), did not correlate with the mutational status of IGHV genes, the stage of CLL, the presence of TP53 and SF3B1 mutations. The relative expression level of the SORL1-Δ2 transcript was 14.1 ± 6.04. SORL1-Δ2 transcript expression higher than the median was more common in patients with stage C (p = 0.001), and in patients with unmutated IGHV genes was associated with a negative course of CLL (median of overall survival was 9 months versus 61 months at low expression level of SORL1-Δ2). The expression levels of the main and alternative transcripts of the SORL1 gene in CLL patients of the main group and the group of comparison did not differ. Preliminary data suggest that a significant increase in the expression of the alternative SORL1-Δ2 transcript in CLL patients with unmutated status of IGHV genes can be considered as a marker of an extremely negative prognosis. Field of application: radiobiology, hematology. Publications - 3 articles, information technology. Product Description popup.authors Abramenko Iryna V Bilous Nadiia I Martina Zoya V popup.nrat_date 2022-03-09 Close