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Information × Registration Number 0222U000734, 0120U103332 , R & D reports Title Development of the technology for obtaining of recombinant bioconjugates for regenerative medicine and study of their properties popup.stage_title Head Kordyum Vitaliy A., Доктор біологічних наук Registration Date 18-01-2022 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  As a result of the second stage of the project, genetic constructs encoding recombinant sHB-EGF with mutations in the heparin-binding domain were obtained for their efficient expression in the prokaryotic synthesis system. This provided an average protein yield: GST-sHB-EGF (norm) - 1.9 mg / ml, GST-sHB-EGF (mut) - 3 mg / ml, GST-sHB-EGF (del) - 2.8 mg / ml ml per liter of bacterial culture. Recombinant hSDF-1α (his) was also obtained in soluble form and it was proved that the obtained protein is able to enhance the migration of human peripheral blood leukocytes. For further immobilization of proteins, a carrier was obtained - water-soluble polysaccharide chitosan (poly-glucosamine) from natural polysaccharide chitin (poly-N-acetylglucosamine).New reagents for fluorescent labeling of biomolecules in aqueous medium based on water-soluble coumarin derivatives were also obtained. They were used to obtain labeled D-glucosamines - potential reagents for fluorescent imaging of cells. Product Description popup.authors Irodov Dmytro M. Gorbatiuk Oksana B. Dubey Igor Ya. Dubey Larisa V. Kolybo Denys Volodymyrovych Kordium Vitalii Arnoldovich Krynina Olha I. Kuziv Yaroslav B. Negrutska Valentina V Pokholenko Yanina O. Rymar Svitlana Yu. Siromolot Andriy A. Toporova Olena K. popup.nrat_date 2022-03-09 Close