Registration Number
0222U000734,
0120U103332
, R & D reports
Title
Development of the technology for obtaining of recombinant bioconjugates for regenerative medicine and study of their properties
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Head
Kordyum Vitaliy A., Доктор біологічних наук
Registration Date
18-01-2022
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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As a result of the second stage of the project, genetic constructs encoding recombinant sHB-EGF with mutations in the heparin-binding domain were obtained for their efficient expression in the prokaryotic synthesis system. This provided an average protein yield: GST-sHB-EGF (norm) - 1.9 mg / ml, GST-sHB-EGF (mut) - 3 mg / ml, GST-sHB-EGF (del) - 2.8 mg / ml ml per liter of bacterial culture. Recombinant hSDF-1α (his) was also obtained in soluble form and it was proved that the obtained protein is able to enhance the migration of human peripheral blood leukocytes. For further immobilization of proteins, a carrier was obtained - water-soluble polysaccharide chitosan (poly-glucosamine) from natural polysaccharide chitin (poly-N-acetylglucosamine).New reagents for fluorescent labeling of biomolecules in aqueous medium based on water-soluble coumarin derivatives were also obtained. They were used to obtain labeled D-glucosamines - potential reagents for fluorescent imaging of cells.
Product Description
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Irodov Dmytro M.
Gorbatiuk Oksana B.
Dubey Igor Ya.
Dubey Larisa V.
Kolybo Denys Volodymyrovych
Kordium Vitalii Arnoldovich
Krynina Olha I.
Kuziv Yaroslav B.
Negrutska Valentina V
Pokholenko Yanina O.
Rymar Svitlana Yu.
Siromolot Andriy A.
Toporova Olena K.
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2022-03-09
