Search academic texts

Information × Registration Number 0222U000926, 0120U100412 , R & D reports Title To develop modern precision methods of acute myeloid leukemia diagnosis using immunophenotypic, epigenetic and molecular markers of leukemic stem cells (шифр: 2.2.5.431) popup.stage_title Head Gluzman Danylo F., Доктор медичних наукDumanskyi Yurii , Доктор медичних наук Registration Date 21-01-2022 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2 The study of the immunophenotype of leukemic stem cells (LSC) was performed in different clinical and prognostic variants of acute myeloid leukemia (AML). It is shown that the subclone of LSC with phenotype CD34+CD38-CD90-CD123+CD117- mostly was determined in myelo-monocytic (M4) and monoblastic / monocytic variants (M5) and was not determined in promyelocytic (M3) and myeloblastic (M2) variants of AML, i.e., types of disease the cellular substrate of which are blast cells with a higher degree of differentiation (CD34-CD38-CD117+/-CD33+CD13+/-). It is obvious that immunophenotypes identified by LSC in M5 and M4 variants of AML more often determine the ineffectiveness of therapy in patients, and the absence of LSC in certain variants of AML, despite the aggressive clinical course of these variants, contributes to faster remission in patients. For the first time the expression of CD150 on promyelocytes in M3 AML was established, which indicates the possibility of its use as a diagnostic marker in the differential diagnosis of this variant. In other variants of AML, in the vast majority of cases, the expression of CD150 is not detected. There is a tendency for the absence of a fraction of CD34+CD38- cells, which can potentially be LSC, in the presence of CD150-positive phenotype in M3 and M5 AML. For the first time, it was found that the expression values of miRNA-29b, -125b and -370 directly correlate with the degree of blast differentiation in patients with AML (r = 0.52, 0.53 and 0.61, respectively). It is shown that a characteristic feature of AML with the LSC phenotype is the high expression of miRNA-29b, -142, -223, as well as a significant decrease in the level of miRNA-125b and -210. The obtained data testify to the expediency of using the identified microRNA panel (-29b, -125b, -142, -210, -223) to improve the differential diagnosis of AML and predict the aggressiveness of the tumor process. Product Description popup.authors Bezhenar Tetiana O. Borikun Tetiana V. Gordiienko Inna M. Zavelevych Mykhailo P. Zadvornyi Taras V. Koval Stella V. Lukianova Nataliya Yu Polishchuk Alona S Ukrayinska Natalia Philchenkov Olexii O. Shlapatska Larysa Mykolayivna Shcherbina Valeriya M Yalovenko Tetyana M. popup.nrat_date 2022-03-09 Close