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Information × Registration Number 0222U001275, 0120U104364 , R & D reports Title Development of methodology for complex biorisk assessment of pesticide-polluted environment for target and non-target organisms popup.stage_title Head Falfushynska Halyna Ivanivna, Доктор біологічних наук Registration Date 26-01-2022 Organization Ternopil National Pedagogical University named after Volodymyr Hnatyuk popup.description2  The purpose of the 2nd stage of the project was to determine the physiological and biochemical reactions of fruit fly D. melanogaster and daphnia D. magna to the effects of herbicides class organophosphates (roundup) and triazines (atrazine) and insecticides which organophosphates (malathion, chlorpyrifos); to expand the existing theoretical ideas on strategies for adapting biota to the damaging effects of pesticides, taking into consideration their individual action. The generalization of the results by multivariate statistics confirms the peculiarities of the response of stress-responsive and detoxifying systems of Drosophila on the one hand as a target organism to the effects of insecticides and daphnia / danio as non-target organisms on the other. The joint localization of daphnia and danio in the plane of graphical display of the results of factor analysis confirms the implementation of common strategies of adaptation in non-target organisms to pesticides, regardless of phylogenetic origin. Chlorpyrifos is the most toxic in terms of the sum of the characteristics of the three organisms, and roundup (environmentally relevant concentrations) and malathion are the least toxic. On the basis of integrated data processing, the indicators that most likely distribute the studied groups were identified: activity of danio lactate dehydrogenase, which primarily rejects the least impacted groups (control and malathion environmentally relevant concentration), the total antioxidant activity of danio, the concentration of carbonyl derivatives and the activity of daphnia catalase, suffered more significant damage. None of the Drosophila parameters was included in the classification tree, which clearly indicates the impossibility of using an organism that is targeted to a certain factor to bioindicate and assess toxicity by a set of biomarkers. A specific set of biomarkers can be used for early detection of the risk of toxic damage to living organisms by pesticides. Product Description popup.authors Babyshen Nataliia Sergiivna Bodnar Oksana I Burdylyuk Nadiia Igorivna Horyn Oksana Igorivna Gusak Victor Vasyljovych Klishch Svitlana Mykolaivna Kovalska Halyna Bogdanivna Strilbytska Olga Myhaijlivna Strutynska Tetyana Romanivna Falfushynska Halyna Ivanivna popup.nrat_date 2022-03-09 Close