Registration Number
0222U001275,
0120U104364
, R & D reports
Title
Development of methodology for complex biorisk assessment of pesticide-polluted environment for target and non-target organisms
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Head
Falfushynska Halyna Ivanivna, Доктор біологічних наук
Registration Date
26-01-2022
Organization
Ternopil National Pedagogical University named after Volodymyr Hnatyuk
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The purpose of the 2nd stage of the project was to determine the physiological and biochemical reactions of fruit fly D. melanogaster and daphnia D. magna to the effects of herbicides class organophosphates (roundup) and triazines (atrazine) and insecticides which organophosphates (malathion, chlorpyrifos); to expand the existing theoretical ideas on strategies for adapting biota to the damaging effects of pesticides, taking into consideration their individual action.
The generalization of the results by multivariate statistics confirms the peculiarities of the response of stress-responsive and detoxifying systems of Drosophila on the one hand as a target organism to the effects of insecticides and daphnia / danio as non-target organisms on the other. The joint localization of daphnia and danio in the plane of graphical display of the results of factor analysis confirms the implementation of common strategies of adaptation in non-target organisms to pesticides, regardless of phylogenetic origin. Chlorpyrifos is the most toxic in terms of the sum of the characteristics of the three organisms, and roundup (environmentally relevant concentrations) and malathion are the least toxic.
On the basis of integrated data processing, the indicators that most likely distribute the studied groups were identified: activity of danio lactate dehydrogenase, which primarily rejects the least impacted groups (control and malathion environmentally relevant concentration), the total antioxidant activity of danio, the concentration of carbonyl derivatives and the activity of daphnia catalase, suffered more significant damage. None of the Drosophila parameters was included in the classification tree, which clearly indicates the impossibility of using an organism that is targeted to a certain factor to bioindicate and assess toxicity by a set of biomarkers. A specific set of biomarkers can be used for early detection of the risk of toxic damage to living organisms by pesticides.
Product Description
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Babyshen Nataliia Sergiivna
Bodnar Oksana I
Burdylyuk Nadiia Igorivna
Horyn Oksana Igorivna
Gusak Victor Vasyljovych
Klishch Svitlana Mykolaivna
Kovalska Halyna Bogdanivna
Strilbytska Olga Myhaijlivna
Strutynska Tetyana Romanivna
Falfushynska Halyna Ivanivna
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2022-03-09
