Registration Number
0222U001887,
0119U000087
, R & D reports
Title
Study of the regenerative potential of stem cells, depending on the age and sex of the donor on the experimental models of the pathology of the nervous system
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Head
Butenko Hennadii M,
Registration Date
04-02-2022
Organization
Institute of genetic and regenerative medicine National Acad.Med.Sci. of Ukraine
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Object - mice line FVB / N, 129 / Sv aged 3-8 months and 13-18 months. Objective - To study the effect of age and sex on the regenerative potential of multipotent mesenchymal stromal cells (MMSC) in experimental pathology of the nervous system and to assess the possibility of its increase by some cellular and endocrine factors. Models: toxic cupric model of demyelination, toxic model of parkinsonism, sciatic nerve injury, introduction of MMSC, melatonin, fibroblast growth factor (FGF-2). Methods: histological, cultural, biochemical, behavioral tests. Used equipment: CO2 incubator "Thermo", laminar biosafety box "Labconco", microscope, electronic scales "Kern & Sohn". Main results: Administration of toxins (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) or cuprizone to mice leads to morphofunctional disorders of the central nervous system (CNS), the severity of which depends on their age and sex. The influence of the age of the donor and recipient on the therapeutic effect of MMSC of different tissue origin in mice with models of CNS pathology was established. The positive effect of the combination of MMSC and melatonin or FGF-2 on the behavior and oxidative stress factors of the brain of mice of different ages with the demyelination model was revealed. Gender features of the positive influence of MMSC on the structure of the injured nerve and the behavior of mice are shown. The results of the work are presented in the form of a report, 9 articles, chapters in 3 foreign monographs, 1 patent.
Product Description
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Kashuk Olga Anatoliivna
Kyryk Vitalii Mykhailovych
Litoshenko Zoya Leonidivna
Lehkostup Liudmyla Anatoliivna
Olar Vitalii Vasilievich
Panteleimonova Tetyana Nikolaevna
Pokholenko Ianina Olexandrivna
Rodnichenko Anzhela Yevhenivna
Savosko Sergii Ivanovich
Toporova Olena Karneliivna
Utko Nataliia Olexandrivna
Kharkevych Iurii Olexandrovich
Chaykovsky Yurii Bogdanovich
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2022-03-09
