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Information × Registration Number 0222U003051, 0120U103276 , R & D reports Title Development and implementation of ILP markers for genetic selection studies of cereals popup.stage_title Head Blume Yaroslav B., Доктор біологічних наук Registration Date 17-02-2022 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2 Bioinformatics search and analysis of sequenced EST sequences of Aegilops tauschii were performed and ILP markers were developed for further use in molecular genetic characteristics of plants. 208 EST sequences of Ae. tauschii were detected in the NCBI database, from which 181 unique sequences were isolated using the online CD-HIT tool. The analysis of all unique EST sequences of Ae. tauschii by comparison with homologous gene sequences of Oryza sativa revealed 14 intron positions and developed 14 ILP markers of Ae. tauschii, which are planned to be used for genetic analysis of intron polymorphism and genotype differentiation cultivated and wild cereals. The probable functional affiliation of ILP markers was established based on BLAST search for homologous sequences for 12 EST sequences of Ae. tauschii in the genome of Oryza sativa. It is the functional affiliation of the homologous rice gene that was defined by us as a functional annotation for the EST sequence of Ae. tauschii and its ILP markers. In general, all ILP markers Ae. tauschii, based on the function of the respective genes, were divided into 4 categories, including household genes, kinase genes, stress-induced protein genes, and transport protein genes. At this stage, it was found that 35% of the developed ILP markers are in the genes of household proteins, 41% - in the genes of kinases, 18% - in the genes of stress-induced proteins and 6% of ILP markers are located in the genes of transport proteins. Amplification of introns in 35 samples of Ae. tauschii and 9 samples of Ae. biuncialis was performed with the newly developed ILP marker Aet_ILP1. It was possible to clearly differentiate the samples at the species level. 17 ILP markers Ae. tauschii have been developed, which can potentially be used for molecular genetic analysis of this species and other cereal species. Product Description popup.authors Bilonozhko Yuliya O. Blume Yaroslav B. Kalafat Liubov O Pirko Yaroslav V. Postovoytova Anastasiya S. Rabokon Anastasiya M. popup.nrat_date 2022-03-09 Close