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Registration Number

0222U003195, 0121U100096 , R & D reports

Title

Mechanisms of regulation of alcoholic fermentation in yeast

Head

Sybirnyi Andrii А., Доктор біологічних наук

Registration Date

21-02-2022

Organization

Institute of Cell Biology of the National Academy of Sciences of Ukraine

Description

 During the implementation of this project, the involvement of peroxis in the fermentation process of xylose in the yeast S. cerevisiae was shown for the first time. A recombinant strain of S. cerevisiae with a deletion of the PEX3 gene encoding a peroxisomal membrane protein was constructed. It was found that increased expression of the PEX34 gene, which encodes a peroxisome integral membrane protein, leads to the formation of larger peroxisomes and increases 1.4 times the production of ethanol from xylose under conditions of alcoholic fermentation. The role of peroxisomal catalase in the process of alcoholic fermentation of xylose in a recombinant strain of S. cerevisiae was first identified. Deletion of the CTA1 gene led to a limitation of biomass growth and a 2-fold reduction in ethanol production from xylose compared to the original strain. The effect of increasing the expression of the alcohol oxidase gene AOX1, the main enzyme peroxisom, on the production of ethanol from xylose and glycerol in the yeast Ogataea polymorpha under conditions of alcoholic fermentation was studied. It was found that the recombinant strain with enhanced expression of the AOX1 gene is characterized by increased ethanol production from xylose and glycerol. Recombinant strains of O. polymorpha with enhanced co-expression of AOX1, TAL1 and TKL1 genes are able to produce 30% more ethanol from xylose during 38 h of alcoholic fermentation at 37 ° C compared to the original strain. Strains of O. polymorpha with impaired peroxis biogenesis were obtained, in particular pex3 mutants that do not form integral peroxisomes and pex11 mutants with impaired peroxisome proliferation, as well as strains with enhanced expression of the PEX11 gene.

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Vasylyshyn Roksolana V.
Dmytruk Kostyantyn Vasylovych
Zazulya Anastasiya Z.
Kurylenko Olena Oleksandrivna
Semkiv Marta Vitaliyivna
Fedorenko Victor О.