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Information × Registration Number 0222U003242, 0117U000848 , R & D reports Title Low temperature preservation of stem cells encapsulated to three-dimensional structures popup.stage_title Head Petrenko Oleksandr Yu., Доктор біологічних наук Registration Date 22-02-2022 Organization Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description2  The aim of the study was to evaluate the effect of low-temperature preservation and hypothermic storage of mesenchymal stem cells (MSCs) and neural stem / progenitor cells (NC) in three-dimensional structures on their viability, morphological and functional properties, realization of proliferation and differentiation potential in monolayer and 3D culture and following experimental transplantation. Materials and methods. Cytofluorimetric, spectrophotometric, spectrofluorimetric, biochemical, immunocytochemical, histological methods, volumetry, physical and mathematical modeling, in vivo microscopy, cell culture, cryopreservation, experimental transplantation were used. Results of the research. Screening of three-dimensional structures of different chemical nature as carriers for MSC culture was performed. Chitosan matrices with a high degree of deacetylation and chitin carriers obtained after demineralization of natural sponges of the Verongida division supported normal cell adhesion and proliferation. Scaffolds based on collagen and its derivatives were safe and biocompatible for 3D culture of MSCs. To assess the reparative potential of MSCs, a modification of the full-layer excisional skin wound model in mice that prevents contraction was developed. It was shown that cryopreserved MSCs embedded in plasma-based hydrogel promoted the process of wound healing similar to non-cryopreserved MSCs. MSCs in 3D scaffolds are more sensitive to the generally accepted method of cryopreservation, which includes slow two-stage freezing under the protection of 10% cryoprotectant DMSO and fetal bovine serum, compared to cells in suspension. Morpho-functional characteristics of MSCs after cryopreservation depend on specific scaffold type. Product Description popup.authors Volkova Nataliya O. Vsevolodska Svitlana O. Grischuk Viktor P. Gubenya Oleksandra S. Mazur Svitlana P. Nemirovska Yulia V. Ochenashko Olga Vadimovna Revenko Olena B. Rogulska Olena Yu. Semenchenko Olga A. Sukhach Oleksandr Mykolayovych Tarusin Dmytro Mykolaiovych Tykhvynska Olga O. Trufanova Natalya A. Cherkashina Daria Viktorivna popup.nrat_date 2022-03-09 Close