Registration Number
0222U003242,
0117U000848
, R & D reports
Title
Low temperature preservation of stem cells encapsulated to three-dimensional structures
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Head
Petrenko Oleksandr Yu., Доктор біологічних наук
Registration Date
22-02-2022
Organization
Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
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The aim of the study was to evaluate the effect of low-temperature preservation and hypothermic storage of mesenchymal stem cells (MSCs) and neural stem / progenitor cells (NC) in three-dimensional structures on their viability, morphological and functional properties, realization of proliferation and differentiation potential in monolayer and 3D culture and following experimental transplantation. Materials and methods. Cytofluorimetric, spectrophotometric, spectrofluorimetric, biochemical, immunocytochemical, histological methods, volumetry, physical and mathematical modeling, in vivo microscopy, cell culture, cryopreservation, experimental transplantation were used. Results of the research. Screening of three-dimensional structures of different chemical nature as carriers for MSC culture was performed. Chitosan matrices with a high degree of deacetylation and chitin carriers obtained after demineralization of natural sponges of the Verongida division supported normal cell adhesion and proliferation. Scaffolds based on collagen and its derivatives were safe and biocompatible for 3D culture of MSCs. To assess the reparative potential of MSCs, a modification of the full-layer excisional skin wound model in mice that prevents contraction was developed. It was shown that cryopreserved MSCs embedded in plasma-based hydrogel promoted the process of wound healing similar to non-cryopreserved MSCs. MSCs in 3D scaffolds are more sensitive to the generally accepted method of cryopreservation, which includes slow two-stage freezing under the protection of 10% cryoprotectant DMSO and fetal bovine serum, compared to cells in suspension. Morpho-functional characteristics of MSCs after cryopreservation depend on specific scaffold type.
Product Description
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Volkova Nataliya O.
Vsevolodska Svitlana O.
Grischuk Viktor P.
Gubenya Oleksandra S.
Mazur Svitlana P.
Nemirovska Yulia V.
Ochenashko Olga Vadimovna
Revenko Olena B.
Rogulska Olena Yu.
Semenchenko Olga A.
Sukhach Oleksandr Mykolayovych
Tarusin Dmytro Mykolaiovych
Tykhvynska Olga O.
Trufanova Natalya A.
Cherkashina Daria Viktorivna
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2022-03-09
