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Registration Number

0222U004533, 0122U002457 , R & D reports

Title

Development of nanostructured optical sensor system for detection of K. pneumonia

Head

Holubnycha Viktoriia M., Кандидат медичних наук

Registration Date

31-08-2022

Organization

Sumy State University

Description

 The object of research is immuno-photo-chemical reactions with the participation of nanocomposite systems immobilized on the surface of glass. The purpose of the work is studying the sensitivity of the photoluminescent platform based on ZnO nanostructures to K. pneumoniae antigens detection. During the implementation of the project, the authors formed theoretical and methodological foundations and improved the technique of creating bioselective layers based on ZnO composites. A method of the bioselective layer forming on the surface of ZnO nanostructures within the limits of covalent binding of specific antibodies has been developed. The efficiency of the deposition of the components of the bioselective layer was evaluated using specific immunological reactions. An experimental setup was created to study the optical properties of composites based on ZnO by the method of photoluminescence spectroscopy, and a developed sample chamber was used to study materials containing K.pneumoniae antigens. Photoluminescence intensity was measured and the transducer output signal measuring was interpreted, which confirmed the possibility of determining K.pneumoniae in the studied sample in an amount exceeding 103 CFU/ml

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Viter Roman V.
Holubnycha Viktoriia M.
Husak Yevheniia V.
Korniienko Viktoriya V.