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Registration Number

0222U004559, 0118U006158 , R & D reports

Title

Development of 2D materials and "smart" sensors for medical and biological purposes.

Head

Dovbeshko Halyna I,

Registration Date

05-09-2022

Organization

Institute of Physics of National Academy of Sciences of Ukraine

Description

During the reporting period, a technique for obtaining protein fibrillar structures as a complex with 2D WS2 materials and carbon nanotubes at various combinations of temperature and pH of the medium was developed. The presence of amyloid fibrils was confirmed by confocal microscopy using a gold nanostructured substrate in order to increase image contrast, as well as by fluorescence spectroscopy with the amyloid-sensitive dye thioflavin T. The obtained structures were characterized, spectral markers of fibril formation were determined. It was shown that fibrillar structures are most effectively formed at high protein concentrations at acidic pH and at low protein concentrations and alkaline pH. It was shown that 2D-WS2 nanoparticles have different effects on the secondary structure depending on the pH of the solution and the concentration of the protein. WS2 can both inhibit and stimulate amyloid formation under various external conditions. It is shown that 2D-WS2 nanoparticles and carbon nanotubes can be used to obtain hybrid structures with specified properties in materials science. Spectroscopic techniques can be used to diagnose diseases associated with the formation of amyloid fibrils.

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Hnatyuk Olena Petrivna
Dovbeshko Galyna Ivanivna
Olenchuk Maryna Volodymyrivna