Registration Number
0222U005245,
0122U200143
, R & D reports
Title
Survival characteristics and mechanisms of inactivation of model viruses during biomass waste processing and resource reuse in the context of biosafety
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Head
Shevchenko Oleksii V., Кандидат біологічних наук
Registration Date
26-12-2022
Organization
Taras Shevchenko National University of Kyiv
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Objects of the research: biological properties, retention of infectivity, epidemic potential of harmful plant viruses during recycling of plant waste.
The aim of the work: study of the biological properties of harmful model viruses and their resistance to environmental factors in biomass waste processing processes.
Methods of the research: virological, serological, molecular, biological and statistical methods.
The project is aimed at researching the survival of plant viruses through their controlled introduction into technological processes of waste processing, as well as biotesting and characterization of introduced model plant viruses to study their ability to infect natural hosts.
The survival characteristics and inactivation mechanisms of model viruses during the processing of biomass waste and the reuse of resources in the context of biological safety were studied as part of the implementation of the SRD. The preservation of serological markers and genetic signatures of model viruses (ToMV, CMV) in the process of anaerobic fermentation was confirmed. For the first time, the preservation of the infectivity of model viruses was proved on the example of tomato mosaic tobamovirus (ToMV) during long-term incubation in anaerobic conditions, which requires attention in the processing of plant waste and the use of renewable resources. It is shown that the artificial introduction of anaerobic microorganisms does not have a significant effect on the stability of the studied model viruses in vitro. It has been proven that potyviruses, such as potato virus Y (PVY), do not survive during anaerobic fermentation and therefore do not pose an epidemic risk when using processed renewable resources.
Product Description
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Budzanivska Iryna Н.
Snigur Halyna O.
Kharina Alla V.
Shevchenko Tatiana M.
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2022-12-26
