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0222U005277,
0120U102039
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Antibiotic resistance mutations in actinobacteria: source of new insights into resistance mechanisms and biotechnological applications
Fedorenko Victor O., Доктор біологічних наук
27-12-2022
Ivan Franko National University of Lviv
The work is focused on molecular genetic mechanisms of induced resistance to antibiotics of different types of action in Streptomyces albus and members of genus Actinoplanes. The subject of the research are genomes of S. albus and Actinoplanes sp. expressing natural or induced in the process of selection antibiotic resistance systems. The goal of the work is to generate and study actinobacterial mutants with altered resistance to antibiotics-inhibitors of transcription, translation and cell wall; to explore the potential of revealed mutations as a biotechnological tool. Microbiological, genetic, molecular biology, chemical, bioinformatic and microscopy methods were used. Phenotypes of rpsL mutants were shown to arise from a complex interaction between point rpsL mutation and the other often uknown ones. A new lincomycin resistance determinant leading to antibiotic overproduction has been revealed; novel rpoB alleles have been also discovered that could confer M. tuberculosis to low rifampicin resistance. A detailed genome study of glycopeptide export and resistance genes across wide range of phylogeny has been elucidated. Our results suggest that glycopeptide (VanHAX) resistance genes in pathogens may come from producer species. Tei3 was shown to function as a constitutive phosphorylase capable of recognizing unglycosylated peptide scaffolds as a ligand. New nanoparticles complexed with antibiotics have been developed.
Ostash Bohdan Omelianovych
Ostash Iryna Stepanivna
Syrvatka Vasyl Yaroslavovych
Yushchuk Oleksandr S.
2022-12-27
Updated: 2025-12-07
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