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Information × Registration Number 0222U005277, 0120U102039 , R & D reports Title Antibiotic resistance mutations in actinobacteria: source of new insights into resistance mechanisms and biotechnological applications popup.stage_title Head Fedorenko Victor O., Доктор біологічних наук Registration Date 27-12-2022 Organization Ivan Franko National University of Lviv popup.description2  The work is focused on molecular genetic mechanisms of induced resistance to antibiotics of different types of action in Streptomyces albus and members of genus Actinoplanes. The subject of the research are genomes of S. albus and Actinoplanes sp. expressing natural or induced in the process of selection antibiotic resistance systems. The goal of the work is to generate and study actinobacterial mutants with altered resistance to antibiotics-inhibitors of transcription, translation and cell wall; to explore the potential of revealed mutations as a biotechnological tool. Microbiological, genetic, molecular biology, chemical, bioinformatic and microscopy methods were used. Phenotypes of rpsL mutants were shown to arise from a complex interaction between point rpsL mutation and the other often uknown ones. A new lincomycin resistance determinant leading to antibiotic overproduction has been revealed; novel rpoB alleles have been also discovered that could confer M. tuberculosis to low rifampicin resistance. A detailed genome study of glycopeptide export and resistance genes across wide range of phylogeny has been elucidated. Our results suggest that glycopeptide (VanHAX) resistance genes in pathogens may come from producer species. Tei3 was shown to function as a constitutive phosphorylase capable of recognizing unglycosylated peptide scaffolds as a ligand. New nanoparticles complexed with antibiotics have been developed. Product Description popup.authors Ostash Bohdan Omelianovych Ostash Iryna Stepanivna Syrvatka Vasyl Yaroslavovych Yushchuk Oleksandr S. popup.nrat_date 2022-12-27 Close