Registration Number
0223U000412,
0121U111587
, R & D reports
Title
Study of the effect of MSCs subpopulations and steroid drugs in experimental model of hypercytokine systemic inflammation
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Head
Nikolskyi Ihor S., д.мед.н.
Registration Date
07-01-2023
Organization
Institute of genetic and regenerative medicine National Acad.Med.Sci. of Ukraine
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It was found that the growth kinetics of fetal musculoskeletal rat MSCs in cell culture is significantly higher than such MSCs from adult thymus according to the number of population doublings per 24 hours (PD) and population doubling time (PDT). Rat fetal musculocutaneous MSCs show a significantly higher clonogenic potential. In vitro induction of MSCs by LPS leads to a significant increase in PD and a decrease in PDT in both cell types, stimulation of the clonogenic potential and contact interaction with lymphocytes. Both types of cells show similar properties in terms of their ability to linear differentiation along osteogenic and adipogenic pathways and contact interaction with lymphocytes. Incubation of fetal musculocutaneous MSCs, but not adult thymic MSCs with LPS, significantly increases the activity of linear differentiation along the osteogenic direction. The addition of dexamethasone to the cell culture neutralizes the effect of LPS. As a result of the combined action of LPS and dexamethasone, the stimulating effects of LPS in relation to the kinetics of MSC growth, the formation of their clonogenic potential and the ability to contact with lymphocytes are canceled.
Product Description
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Nikolska Valentina V
Semenova Yanina-Mariya
Taranukha Lyubov I
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2023-01-07
