Registration Number
0223U000902,
0120U100748
, R & D reports
Title
Investigation of the possibility of modification by astaxanthin of radiation-induced in vitro genome injuries in peripheral blood lymphocytes of individuals with BRCA1 gene mutations.
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Head
Kurinnyi Denys A., Кандидат біологічних наук
Registration Date
21-01-2023
Organization
State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine"
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Object of research: peripheral blood lymphocytes of conditionally healthy individuals and individuals carrying mutations in the BRCA1 gene. Objective: to investigate the effect of astaxanthin on changes in the frequency and spectrum of chromosome aberrations, indicators of DNA damage and the level of apoptosis in blood lymphocytes of BRCA1 gene mutation carriers irradiated at a dose of 1.0 Gy. Research methods: molecular genetic - polymerase chain reaction method; method of comet electrophoresis of individual cells (Comet assay); cytogenetic, statistical. A comparison of the level of genomic instability after administration of astaxanthin in irradiated and non-irradiated peripheral blood lymphocytes of 10 conditionally healthy individuals from the control group and 5 asymptomatic (without realized oncopathology) BRCA1 gene mutation carriers was conducted. Established the effect of astaxanthin in the case of disruption of repair processes due to the presence of the hereditary mutation 5382insC in the BRCA1 gene activates the process of apoptosis, which is the key mechanism for regulating the development of genomic instability in the somatic cells of individuals who are asymptomatic carriers of a mutation in the BRCA1 gene.
Product Description
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Demchenko Olena Mykolaivna
Klymenko Sergiy Viktorovych
Meheda Ludmila Volodymyrivna
Rybchenko Ludmila Anatolivna
Romanenko Maryna Gennadivna
Stefanovych Ganna Volodymyrivna
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2023-01-21
