Звіт по науково-дослідній роботі. Фарат Олег Костянтинович. Безпрецедентні перегрупування оксазагетероциклів як новий метод синтезу ксантенових барвників для біовізуалізації

Information × Registration Number 0223U003774, 0122U201744 , R & D reports Title Unprecedented rearrangements of oxazaheterocycles as a new method of synthesis of xanthene dyes for biovisualization popup.stage_title Head Farat Oleh K., Доктор хімічних наук Registration Date 29-06-2023 Organization Farat Oleh K. popup.description2  The object of research is oxazaheterocyclic systems, xanthene-based dyes, fluorescence in the near-infrared region. The goal of the work is the synthesis and research of compounds that absorb/emit in the range of 650-800 nm. Provided the positive results of biochemical tests are obtained, the synthesized compounds can be offered as photostable, low-toxicity, inexpensive fluorescent probes for laboratory experiments of oncological analyses. New methods of targeted synthesis of fluorescent and pharmacophore materials will be developed. The regularities of the influence of chemical modification of the xanthene fragment, substituents and various solvents on the spectral properties will be established. Important parameters for tumor markers and optical filters will be determined, such as the stability of the binding complexes of fluorescent dyes with cell molecules, photo- and chemical stability in the solution with changes in pH and temperature of the environment, establishing safety for unaffected cells. It is expected that the synthesized fluorophores will combine photo- and chemical stability under pH and temperature changes with low toxicity, and will be capable of modulating their fluorescence. Research methods – chemical synthesis, thin-layer chromatography (TLC), X-ray structural analysis (XRA), infrared spectroscopy (IR) and nuclear magnetic resonance (NMR) spectroscopy on 1H and 13C nuclei, ultraviolet and fluorescence spectroscopy, mass spectrometry and in silico prognostication. As a result of the research, the NTP was obtained: "Methodology for the synthesis of 2-[(E)-2-(9-{[(1E)-(dimethylamino)methylene]amino}-2,3-dihydro-1H-xanthen-4-yl)vinyl]-1,3,3 -trimethyl-3H-indolinium iodide"; "Method of synthesis of new compounds of the xanthene series with radiation in the NIR region." The field of use of work results - C 20.12 Production of dyes and pigments. Product Description popup.authors Farat Oleh K. popup.nrat_date 2023-06-29 Close