Registration Number
0223U004677,
0123U102741
, R & D reports
Title
Biotechnological and pharmacological potential of new antibiotic Je478 with the antimycobacterial activity
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Head
Fedorenko Viktor O., Доктор біологічних наук
Registration Date
29-11-2023
Organization
Ivan Franko National University of Lviv
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Research object – bacterial strain Streptomyces sp. Je 1-332 and biosynthesis of new guanidine containing terpene antibiotic Je478.
Purpose of work – to optimize the Streptomyces sp. Je 1-332 cultivation conditions for improved production of Je478, develop genetic manipulation methods to be used in this strain and identify, clone and express the Je478 biosynthetic genes.
Research methods – general microbiological methods, genome sequencing, assembly and annotation, transposon mutagenesis, directed mutagenesis, bioinformatic analysis, biosynthetic pathways prediction, arranged cosmid library construction, heterologous expression, and liquid chromatography-mass spectrometry.
Results and novelty: The spread of antibiotics resistance among pathogenic microorganism led to establishment of the current situation when some of the bacterial infections could no longer be treated with the available medicines. This is why the search to new antibacterial compounds in of high importance. In the scope of first stage of the project we first: a) performed complete microbiological characterization of new strain Streptomyces sp. Je1-332, the producer of Je478; b) optimized Streptomyces sp. Je1-332 cultivation conditions for improved production of Je478; c) developed a gene cloning system for Streptomyces sp. strain Je 1-332 and its efficiency was demonstrated using integrative vectors containing reporter genes (gusA and rppA); e) sequenced and analyzed the genome of Streptomyces sp. Je1-332, identified genes encoding secondary metabolism; f) constructed arranged cosmid library of Streptomyces sp. Je1-332 genome; g) identified biosynthetic genes for the new class of terpen antibiotics represented by its prototype Je478; h) constructed platform for heterologous production of Je478 in S. albus strain.
Product Description
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Hromyko Oleksandr M.
Ostash Iryna Stepanivna
Rebets Yuriy V.
Samborskyy Markiyan
Syrvatka Vasyl Yaroslavovych
Tistechok Stepan I.
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2023-11-29
