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Registration Number

0223U004969, 0123U103415 , R & D reports

Title

Development of an enzyme kit and a portable biosensor for express assay of creatinine – a marker of acute renal dysfunction

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Head

Dziadevych Serhii V., Доктор біологічних наук

Registration Date

11-12-2023

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

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 The pH sensitivity and stability of transducers based on pH-sensitive field-effect transistors and their functionality were investigated. The effect of ultraviolet treatment on the characteristics of pH-FET electrodes was studied. It is proposed to introduce an additional technological operation - preliminary UV treatment of electrodes to stabilize the characteristics of ISFET (threshold voltage) and the possibility of performing further UV irradiation operations when applying biomembranes to the electrodes, as well as to prevent the influence of sunlight on the characteristics of transistors when used in real field conditions. Two methods of creatine deiminase immobilization on the surface of pH-sensitive field-effect transistors were investigated: in glutaraldehyde vapors and photopolymerization in PVA/SbQ. It is shown that photopolymerization is a more suitable method for immobilization of creatine deiminase.

Product Description

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Arkhypova Valentyna M.
Kykla Olexander L
Shkotova Lyudmyla V.

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2023-12-11