Registration Number
0223U005810,
(0120U100298)
, R & D reports
Title
Investigation of the role of сell signaling second messengers in action of phytohormones and their structural analogs.
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Роль діацилгліцеролкіназ зі зміненою експресією генів ряду їх ізоферментів в регуляції метаболізму клітин за дії поліамінів.
Head
Kravets Volodymyr S., Доктор біологічних наук
Registration Date
31-12-2023
Organization
V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry of the National Academy of Sciences of Ukraine
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The project is devoted to understanding of the nature of primary cellular reactions to a number of phytohormones, polyamines and the role of phosphatidylinositol 4-kinases (PI4K) and EDS1 (ENHANCED DISEASE SUSCEPTIBILITY 1) proteins in production of cell signaling second messengers during the regulation of plant metabolism. Solving these goals anticipates the investigation of molecular mechanisms that transduce a phytohormonal signals in plant cells inducing a range of consequtive changes in intensity and direction of metabolic pathways leading to plant adaptation. Special attention will be paid to examination of the role of phosphatidylinositol 4-kinases and EDS1 proteins in formation of cell signaling second messengers in response to a range of phytohormones with application of transgenic Arabidopsis thaliana plants with mutation in phosphatidylinositol 4-kinases (PI4K) genes: pi4kβ1β2, sid2pi4kβ1β2, pi4kb1b2sid2eds1. Investigations of the role of phospholipases with changed expression of their isoforms are also planned in regulation of key enzymes of proline metabolism in response to polyamines. Analysis of specific responses of serine-rich peptides SCOOP is envisaged in regulation of cellular metabolism of Arabidopsis thaliana plants. Changes in phospholipid profile in Arabidopsis thaliana will be investigated during plant response to elicitors and hemi-biotrophic fungal infections with the help of genetically-inserted fluorescent markers. Investigations of the role of diacylglycerol kinases with changed expression of their isoforms will be also conducted in regulation of cell metabolism in response to polyamines.
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The results were obtained on the involvement of specific diacylglycerol kinase isozymes in the realization of the biological action of polyamines in the process of forming resistance to salt stress. The levels of markers of lipid peroxidation, hydrogen peroxide and superoxide, reduced glutathione, proline levels, activity of proline dehydrogenase and pyrroline 5-carboxylate synthetase enzymes, formation of the secondary mediator phosphatidic acid in transgenic Arabidopsis thaliana plants were studied by thin-layer chromatography of fluorescently labeled lipids. The data obtained indicate that the isozymes of diacylglycerol kinases such as DHA2 and DHA3 are involved in the realization of the action of polyamines in Arabidopsis thaliana plant cells in the formation of resistance to salt stress in the processes of modulating the formation of phosphatidic acid, a secondary mediator of plant cell signaling systems, regulating the balance of reactive oxygen species and levels of osmoprotectors. The obtained data will allow to form practical bases for modern technologies of crop cultivation in areas of risky farming.
Product Description
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Bukhonska Yaroslava К.
Derevyanchuk Michael V.
Karakhim Sergii O
Kolesnikov Yaroslav S.
Kretynin Serhiy V.
Nikolayevska Valentyna І.
Pokotylo Igor V.
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2023-12-31
