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Information × Registration Number 0224U000173, 0121U100569 , R & D reports Title Creation of androgenic double spring barley haploid via intervariety and interspecies crosses and enrichment of trait collections due to introgressive lines popup.stage_title Head Bilynska Olena V., Кандидат біологічних наук Registration Date 03-01-2024 Organization Institute of Plant Breeding named after V. Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine popup.description2 Study object  morphogenesis in spring barley anther culture in vitro, genetic diversity of spring barley and H. spontaneum in biological and valuable agronomic traits. Purpose  to determine androgenetic capacities of H. spontaneum accessions and spring barley varieties, which are sources of high protein content, to evaluate the genetic variability of H. spontaneum in terms of protein content and vernalization requirement, to improve the method of plant regeneration in anther culture in vitro, to obtain intervariety and interspecies crosses, doubled haploid by improved technique. Methods  anther culture in vitro, light microscopy, Kjeldal digestion, infrared spectroscopy, artificial hybridization, variation statistics, ANOVA. Theoretical and practical results  Medium for plant regeneration has been developed and optimal time for transfer of morphogenic structures on regenerative medium has been determined. Spring barley varieties combining high protein content with high capacity for androgenesis in vitro were selected. From 18 F1-F2 hybrid populations, including 15 interspecies ones, 1,122 green plants were regenerated in anther culture in vitro and 53 doubled haploid lines were obtained. "Method for Determination of Starch Type in Barley Seeds" has been elaborated (No a2023 04304). The results can be used in experiments in plant biotechnology, in particular in anther culture in vitro, and in breeding programs aimed to produce spring barley starting materials with improved grain quality Product Description popup.authors Bohuslavskyi Roman L. Sheliakina Tetiana A. popup.nrat_date 2024-01-03 Close
R & D report
Creation of androgenic double spring barley haploid via intervariety and interspecies crosses and enrichment of trait collections due to introgressive lines
Head: Bilynska Olena V.. Creation of androgenic double spring barley haploid via intervariety and interspecies crosses and enrichment of trait collections due to introgressive lines. (popup.stage: ). Institute of Plant Breeding named after V. Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine. № 0224U000173
1 documents found

Updated: 2026-03-26


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