Registration Number
0224U000173,
(0121U100569)
, R & D reports
Title
Creation of androgenic double spring barley haploid via intervariety and interspecies crosses and enrichment of trait collections due to introgressive lines
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Створення андрогенних подвоєних гаплоїдів ярого ячменю на основі міжсортових та міжвидових схрещувань та поповнення ознакових колекцій за рахунок інтрогресивних ліній
Head
Bilynska Olena V., Кандидат біологічних наук
Registration Date
03-01-2024
Organization
Institute of Plant Breeding named after V. Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine
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Purpose - to create lines of spring barley double haploids via intervariety and interspecies crosses with H. spontaneum (C. Koch.) Thell. using by anther culture in vitro and to enrich the gene pool of spring barley with introgressive lines
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Study object morphogenesis in spring barley anther culture in vitro, genetic diversity of spring barley and H. spontaneum in biological and valuable agronomic traits. Purpose to determine androgenetic capacities of H. spontaneum accessions and spring barley varieties, which are sources of high protein content, to evaluate the genetic variability of H. spontaneum in terms of protein content and vernalization requirement, to improve the method of plant regeneration in anther culture in vitro, to obtain intervariety and interspecies crosses, doubled haploid by improved technique. Methods anther culture in vitro, light microscopy, Kjeldal digestion, infrared spectroscopy, artificial hybridization, variation statistics, ANOVA. Theoretical and practical results Medium for plant regeneration has been developed and optimal time for transfer of morphogenic structures on regenerative medium has been determined. Spring barley varieties combining high protein content with high capacity for androgenesis in vitro were selected. From 18 F1-F2 hybrid populations, including 15 interspecies ones, 1,122 green plants were regenerated in anther culture in vitro and 53 doubled haploid lines were obtained. "Method for Determination of Starch Type in Barley Seeds" has been elaborated (No a2023 04304). The results can be used in experiments in plant biotechnology, in particular in anther culture in vitro, and in breeding programs aimed to produce spring barley starting materials with improved grain quality
Product Description
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Bohuslavskyi Roman L.
Sheliakina Tetiana A.
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2024-01-03
