Registration Number
0224U000174,
(0123U100486)
, R & D reports
Title
Development of a test system for screening the stress-protective effect of new physiologically active substances on grain cereals
popup.stage_title
Розроблення тест-системи для скринінгу стрес-протекторної дії нових фізіологічно активних речовин на зернові злаки
Head
Kolupaiev Yurii Ye., Доктор біологічних наук
Registration Date
03-01-2024
Organization
Institute of Plant Breeding named after V. Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine
popup.description1
Creation of a test system for evaluating the effectiveness of the stress-protective action of physiologically active substances, primarily new classes and groups, based on determining their effect on growth, integral indicators of oxidative damage, and the functioning of antioxidant and osmoprotective systems under the influence of stress factors on grain cereals of different genotypes in the early stages of development
popup.description2
Study object - methodical approaches to evaluation of anti-stress effects of physiologically active substances (PASs) on plants. Purpose - to create a test for evaluation of the anti-stress action of physiologically active substances, primarily new classes and groups, based on determination of their impact on growth, integral indicators of the development of oxidative damage and the functioning of antioxidant and osmoprotective systems under the influence of stressors on cereals of different genotypes during the early stages of development. Methods - physiological, biochemical, mathematical and statistical. Theoretical and practical results - Methods for investigations of resistance of cereal seedlings to hyperthermia and osmotic stress and assessment of susceptibility of cereals to physiologically active substances with anti-stress activities have been developed; models explaining the mechanisms of anti-stress effects of melatonin and γ-aminobutyric acid (GABA) on cereals have been justified. A possibility of using roots of intact seedlings as a model object for studying the mechanisms of anti-stress action of PASs by inhibitory method has been demonstrated. Scientific and methodological guidelines "Evaluation of Anti-Stress Effects of Physiologically Active Substances on Cereals" have been prepared. Novelty - the scientific and technical products are better than the existing in Ukraine analogs; the developed methodical approaches were shown to be highly efficient in elucidating the mechanisms of the anti-stress effects of melatonin and GABA on plants and allowed for the first time to investigate the effects of functional interactions of reactive oxygen species and calcium as signal mediators in the action of the specified compounds. Scope of implementation - the developed methods are intended for scientific studies of the nature and mechanisms of the action of physiologically active substances on plants at institutions of NAS of Ukraine and NAAS of Ukraine
Product Description
popup.authors
Kokorev Oleksandr Ihorevych
Riabchun Nataliia I.
Suprun Oleh H.
Shakhov Ivan V.
Yastreb Tetiana O.
popup.nrat_date
2024-01-03
