Registration Number
0224U000305,
(0123U103641)
, R & D reports
Title
Characteristic of microorganisms from biotopes of terrestrial communities of the maritime Antarctic
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Характеристика мiкроорганiзмiв з бiотопiв наземних угруповань Мopської Антарктики
Head
Hnatush Svitlana O., Кандидат біологічних наук
Registration Date
05-01-2024
Organization
Ivan Franko National University of Lviv
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Study of different groups of microorganisms from plant communities from bird nesting sites and the activity of Antarctic marine mammals, isolation of pure cultures and determination of their physiological and biochemical properties, ability to synthesize biologically active substances and mobilize phosphorus and ferrum compounds, nitrogen fixation and molecular genetic characteristic of strains. According to the results of the work, it is planned to deposit 2 strains of microorganisms in the Depository of microorganisms of the D. K. Zabolotny Institute of Microbiology and Virology of the NAS of Ukraine.
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The total quantity and quantity of microorganisms of different ecological trophic groups were determined in the samples of terrestrial plant communities of the maritime Antarctic. Rhizosphere and endophytic microbiota were studied and isolates of endophytic, epiphytic and rhizosphere microorganisms of Deshampcia antarctica E. Desv. and Colobanthus quitensis (Kunth) Bartl. were selected, including able to synthesize biologically active substances. 100 isolates from the rhizosphere zone and 20 isolates from the endosphere zone of D. antarctica were isolated. 90 isolates from the rhizosphere zone and 35 isolates from the endosphere zone of C. quitensis were isolated. Their morphological, cultural, physiological and biochemical characteristics were studied. The isolates were screened for their ability to synthesize indole-3-acetic acid, produce siderophores, and stimulate wheat growth. When wheat seeds were treated with isolates from the rhizosphere zone, seed germination increased, root length and leaf area increased, and chlorophyll content in wheat leaves increased. 4 isolates of microorganisms characterized by the best growth-stimulating properties were selected. According to the results of the study of their characteristics and sequencing of the 16S rRNA gene, the isolates were identified as representatives of the genera Lysinibacillus, Microbacterium, Arthrobacter and Pseudomonas. Nucleotide sequences of the 16S rRNA gene have been deposited in the GenBank database. Two strains: Lysinibacillus sp. Ekm8 and Microbacterium sp. EO1, deposited in the Depository of microorganisms of the D. K. Zabolotny Institute of Microbiology and Virology of the NAS of Ukraine. Certificates about deposit of 2 strains: Lysinibacillus sp. IMV B-8081 and Microbacterium sp. IMV B-8080, dated November 28, 2023, have been received. The strains are promising for use in the development of microbial preparations to improve seed germination and stimulate plant growth.
Product Description
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Komplikevych Solomiya Ya.
Maslovska Olga D.
Moroz Oksana M.
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2024-01-05
