Registration Number
0224U000380,
(0121U100472)
, R & D reports
Title
Development of intraoperative diagnosis method for malignant tumors determining using fluorophore-conjugated antibodies to carcinoembryonic antigens
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In vivo дослідження флюорофор-кон'югованих анти-CEACAM антитіл.
Head
Lyndin Mykola S., Кандидат медичних наук
Registration Date
05-01-2024
Organization
Sumy State University
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Development of a technique for intraoperative photodynamic imaging in the near-infrared region of the spectrum of malignant tumors of the main localizations using fluorophore-conjugated antibodies to cancer-embryonic antigen molecules.
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The object of research is malignant tumors of the main localizations. The subject of research is the immunophenotype of malignant tumor cells, molecular features of the interaction of fluorophore-conjugated antibodies with cancer cells in vitro and in vivo, the effectiveness of fluorescence-guided surgery in the visualization of malignant neoplasms. The aim of the work is to create a technique of intraoperative photodynamic imaging in the near infrared region of the spectrum of malignant tumors of the main localizations using fluorophore-conjugated antibodies to molecules of cancer-embryonic antigen. To achieve this goal, the following tasks were identified: investigate the immunological profile of malignant tumors regarding CEACAM, establishing their differences from the immunophenotype of unaffected tissues; develop comprehensive fluorophore-conjugated complexes with anti-CEACAM antibodies and determine their in vitro level of selectivity and stability; examine the in vivo intraoperative diagnostic effectiveness of fluorophore-conjugated antibodies in modeling malignant tumors in animals; formulate protocols for intraoperative diagnosis of malignant tumors and provide recommendations for their practical medical application. Research methods include histological and immunofluorescent studies, the creation of fluorophore-conjugated anti-CEACAM antibodies, in vivo experiments, and mathematical methods. The studies aim to establish the immunophenotype of tumor tissue, determine the uniqueness of their receptor profile, synthesize highly selective fluorescent messengers, and test their effectiveness in experiments
Product Description
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Zolotareva Vira H.
Kravchenko Yaroslav O.
Lyndin Mykola S.
Lyndina Yuliia M.
Oleshko Tetiana B.
Sikora Vladyslav V.
Sikora Kateryna O.
Sikora Yana V.
Smorodska Olga M.
Stroy Elizaveta A.
Tsepochko Dariya H.
Yanko Illya K.
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2024-01-05
