Registration Number
0224U000537,
(0121U108989)
, R & D reports
Title
Study of cryoprotective activity of the media, containing nanosized particles
popup.stage_title
Дослідження кріозахисної активності середовищ, що містять нанорозмірні частки
Head
Chyzhevskyi Viktor V., Кандидат біологічних наук
Registration Date
09-01-2024
Organization
Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
popup.description1
Research aim is to determine the physical properties and cryoprotective activity of the media, containing cryoprotectants and nanosized particles (nanocrystalline cerium dioxide and hydrated fullerene C60) for cryopreservation and storage of biological objects under moderately low temperatures.
popup.description2
The objects of research are nanocrystalline cerium dioxide (NDC), fullerene C60, erythrocytes, oysters Crassostrea gigas, yeast Saccharomyces cerevisiae, DMSO, fibroblast-like cells. The purpose of the work is to determine the physical properties and protective effect of media containing DMSO and/or nano-sized particles, nanocrystalline cerium dioxide and hydrated C60 fullerene, for cryopreservation and storage of biological objects at low temperatures. Cryobiological, thermoplastic deformation, spectrophotometric, photocolorimetric, microscopic, biochemical, cultural methods were used in the work. It was established that NDC in concentrations of 0.02 - 2.1 g/l leads to significant changes in the phase-structural transformations of cryoprotective media containing DMSO, increases the glass transition temperature and reduces/levels off the degree of recrystallization processes in the experimental samples. NDC as a part of the protective medium has a multidirectional effect on the character of the generation of blood lipoperoxidation products at different times of hypothermic storage, which may indicate the ability of nanoparticles to act on the membrane of erythrocytes during hypothermic storage. Pretreatment of oysters in seawater with the addition of fullerene C60 slows down the activation of free radical oxidation processes of biopolymers and reduces the accumulation of lipid products in the tissues of molluscs. Fibroblast-like cells, cryopreserved with the addition of 10 μg of NDC to 0.5 ml of cryoprotective medium containing 10%, 7% and 5% DMSO retain viability and the ability to recover in monolayer culture. It has been proven that fullerene C60 affects the spectral characteristics of the intrinsic luminescence of yeast cells S. cerevisiae and changes the organoleptic profile of wine in winemaking technologies.
Product Description
popup.authors
Polyakova Ganna L.
Falko Oksana V.
popup.nrat_date
2024-01-09
