Search academic texts

Information × Registration Number 0224U000734, 0123U101591 , R & D reports Title Study of modification of radiation-induced and tumor-induced genome damage in peripheral blood lymphocytes of individuals with glioblastoma popup.stage_title Head Kurinniy Denys A., Кандидат біологічних наук Registration Date 11-01-2024 Organization State institution "National Scientific Center of Radiation Medicine of the National Academy of Medical Sciences of Ukraine" popup.description2   The object of the study is the metaphase plate, genomic DNA, peripheral blood lymphocytes in the state of apoptosis of conditionally healthy individuals and patients with glioblastoma. The purpose of the work is to investigate the effect of glioblastoma on changes in the frequency and spectrum of chromosome aberrations, indicators of DNA damage and the level of apoptosis in non-irradiated peripheral blood lymphocytes of patients with glioblastoma. Research methods: molecular genetic (comet electrophoresis of individual cells, "Comet assay"); cytogenetic, statistical. The level of genomic instability in peripheral blood lymphocytes of 10 conditionally healthy individuals (control group) and 20 patients with verified glioblastoma (examination group) was compared. The features of the influence of glioblastoma on the development of genomic instability were determined: the levels of cytogenetic (frequency of chromosomal aberrations) and molecular genetic (frequency of DNA damage and apoptotic activity) indicators in peripheral blood lymphocytes of conditionally healthy individuals and patients with glioblastoma were determined. A statistically significant increase in the levels of chromosomal aberrations, DNA damage and the frequency of cells in the state of apoptosis in the culture of peripheral blood lymphocytes in patients with glioblastoma compared to the corresponding indicators of conditionally healthy individuals was established. Thus, as a result of performing the NDR stage, it was established the presence of a tumor-induced witness effect in peripheral blood lymphocytes of patients with verified glioblastoma. Under the influence of transformed tumor cells, there is an increase in genomic instability in witness cells (lymphocytes of peripheral blood). Activation of the apoptosis process is a key mechanism for regulating the development of genomic instability in somatic cells. Product Description popup.authors Hlavatskyi Oleksandr Yakovich Demchenko Olena Mykolaivna Zemskova Oksana Volodymyrivna Meheda Lyudmila Volodymyrivna Romanenko Marina Gennadiyevna Stefanovych Hanna V. popup.nrat_date 2024-01-11 Close