Registration Number
0224U001528,
0121U107613
, R & D reports
Title
Investigation of the mechanisms of beta-lactam antibiotics resistance formation in microorganisms of different taxonomic groups
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Head
Skliar Nadiia I., Кандидат медичних наук
Registration Date
25-01-2024
Organization
State Institution "I. Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine"
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The objects of the study: 29 strains of S. aureus and 45 strains of E. coli.
The aim of the study: study of the bacterial resistance to β-lactam antibiotics formation in historical isolates, obtained in different periods of antibiotic use.
Methods: microbiological, molecular genetic, analytical, mathematical and statistical.
mecA and blaZ genes play a dominant role in encoding beta-lactamase production in S. aureus strains, and blaCTX-M genes in E. coli strains. Screening of historical isolates for presence of mecA gene in 29 S. aureus strains and the blaСТХ-M gene in 45 E. coli strains was performed, and the correspondence of the genotype to the phenotypic characteristics of antibiotic sensitivity was compared. In vitro adaptation of staphylococci to benzylpenicillin, Escherichia to ceftazidime was carried out. It was shown that all the studied strains, regardless of the period of their isolation, were able to adapt to increasing concentrations of the antibiotic, however, the strains isolated in the modern period were characterized by faster rates of resistance formation and adapted to higher concentrations of the antibiotic than the strains isolated in the pre- and metantibiotic periods. It was established that adaptation to increasing concentrations of antibiotics was accompanied by clonial polymorphism, inhibition of growth kinetics, decrease in the number of CFUs, changes in the biochemical profile, and formation of cross-resistance to antibiotics of other groups.
Product Description
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Krestetska Svetlana L
Peretyatko Оlena
Sklyar Nadiya
Holodna Tetiana V
Chyhyrynsky Ruslan
Jagnuk Julia A
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2024-01-25
