Registration Number
0224U002539,
(0121U100496)
, R & D reports
Title
Investigate the degree of individual QTL genes influence on the development level of meat productivity genotypes of the created sheep new gene pool
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Дослідження ступеню впливу окремих QTL-генів на рівень розвитку м’ясної продуктивності генотипів створюваного нового генофонду овець
Head
Iovenko Vasyl M., Доктор сільськогосподарських наук
Registration Date
18-02-2024
Organization
MF Ivanov Askania-Nova Institute of Steppe Animal Husbandry - National Scientific Breeding and Genetics Center for Sheep Breeding
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Identification of genes influencing on the sheep productivity level and these genes polymorphism level; study of the individual QTL- loci influence on the growth and development of the Ascanian selection sheep; determination the correlations types between certain genes and the level of quantitative and qualitative characteristics sheep development of the created gene pool. Obtaining new knowledge on the molecular mechanisms of the biodiversity formation the breeds, types and lines of sheep during their creation and improvement.
The research plan assumes typing of different breeds Ascanian selection sheep using DNA technologies to assess the populations genetic structure by genes for quantitative traits associated with productivity indicators. At the final stage, recommendations will be developed on the use of the revealed patterns to increase the selection efficiency of new gene pools being created.
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The aim of the work is to identify genes affecting to the sheep level productivity and these genes polymorphism level; study the influence of individual QTL - loci on the Ascanian breeding sheep growth and development; determination of the correlation’s types between the identified genes and the quantitative and qualitative sheep traits development level of the gene pool being created. The object of research is the genes polymorphism, those involved in the sheep development of certain quantitative and qualitative productive traits. Research methods are molecular genetic (PCR-PDRF), population genetic.
In accordance to the results of PCR-PDRF analysis by the CAST, GH and CLPG genes, were investigated the sheep populations genetic structures of the Ascanian Meat-and-Wool breed (AMW), the Ascanian Fine-Fleeced breed (AFF) and their hybrids with the Merinolandschaf and Texel breeds, as well as the growth and development of different genotypes young animals. It was established that CAST and GH genes are polymorphic, and CLPG is monomorphic.
According to the growth hormone gene, the genotyped AFF animals and crossbreeds are characterized by a high concentration of the G allele (0.525 – 0.714), and for the calpastatin gene – the M allele (0.719 – 0.983). Population genetic parameters (Ho, He, Fis, PIC, χ2) were calculated by CAST and GH genes. Young sheep with GH GG and CAST MM genotypes are characterized by a high level of live weight development compared to their peers.
According to the research results, a method of assessment, selection and forecasting of the sheep meat productivity level development at an early age has been developed.
Product Description
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Huzevatyi Oleh Ye.
Dzitsiuk Valentyna V.
Pysarenko Nadiia B.
Skrepets Kostiantyn V.
Yakovchuk Hanna O.
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2024-02-18
