Registration Number
0224U003101,
0123U101850
, R & D reports
Title
Determination of the role of molecular genetic predictors in the early diagnosis and prognosis of malignant tumor diseases of the urinary system
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Head
Harbuzova Viktoriia Yu., Доктор біологічних наук
Registration Date
09-04-2024
Organization
Sumy State University
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During the first stage of the project, blood samples were collected from healthy donors and patients with malignant tumors of the urinary system. Patients were interviewed on key points of their life and disease history. We performed a histological examination of neoplastic tissues to determine the histological type of tumors, the degree of their differentiation, local and distant spread, the degree of lymphovascular invasion, and the qualitative and quantitative characteristics of the tumor microenvironment. The immunohistochemical study revealed the peculiarities of the expression of prognostically important apoptosis macrophages (p53, bcl-2) and proliferative cell antithesis (Ki-67).
The obtained results demonstrate the dependence of the immunoprofile of urinary tract carcinomas on their histologic variant and degree of differentiation. At the same time, the expression patterns of proapoptotic markers (p53 and bcl-2) and indicators of neoplastic cell proliferative activity (Ki-67) can serve as prognostic markers for local and distant spread of tumors and their ability to lymphovascular invasion. We have also shown that with the progression of the neoplastic process, not only the parinchymatous component of neoplasms but also its stromal component is subject to qualitative and quantitative variations. At the same time, the tumor microenvironment is also a reliable prognostic indicator of carcinoma progression.
The results obtained are of direct practical relevance, as they confirm the involvement of certain proteins regulating key pathogenic links of apoptosis in the initiation and progression of malignant tumors. These conclusions can be used in the diagnostic analysis of biopsy and autopsy material to identify cells that have undergone neoplastic transformation, determined the degree of their differentiation, and monitored the progression of urinary system tumors
Product Description
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Ivanova Anna S.
Biesiedina Antonina A.
Borshchenko Valeriia Ya.
Harbuzova Viktoriia Yu.
Demenko Maryna M.
Diadchenko Olha S.
Kryvonos Tetiana V.
Kryvorotenko Ruslan I.
Lebed Liubov P.
Levchenko Zoia M.
Mykhailichenko Halyna H.
Obukhova Olha A.
Psariova Valentyna H.
Romaniuk Anatolii M.
Sikora Kateryna O.
Skibchyk Yuliia A.
Stroi Elizaveta A.
Chumachenko Yaroslav D.
Shkatula Pavlo Yu.
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2024-04-09
