Registration Number
0224U032404,
(0120U103183)
, R & D reports
Title
Creation of the library of monoclonal antibodies to the alphaC-region of fibrinogen for study of its role in the fibrin polymerization and for the development of immunodiagnostics.
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Створення бібліотеки моноклональних антитіл до альфаС-регіону фібриногену з метою вивчення його ролі в полімеризації фібрину та для розробки імунодіагностикумів
Head
Platonova Tetyana M., Доктор біологічних наук
Registration Date
06-10-2024
Organization
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine
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Obtaining and immunochemical characterization of new monoclonal antibodies to the αC-region of the fibrin(ogen) molecule for their possible further use in studying of the structure and function of the αC-regions of the fibrin(ogen) molecule during its polymerization, as well as for the development of immunodiagnostics.
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By the method of a limited proteolysis using protease from the venom of Gloydius halys halys, a fragment of the Aα-chain of the fibrin(ogen) molecule Aα414-610 was obtained, which was characterized using mass spectrometry, gel electrophoresis, and western blot analysis. The resulting fragment was used to immunize mice, and stable hybridomas were obtained, capable of producing monoclonal antibodies to various sections of the αC-region of fibrin for the purpose of creating hybridomas. Monoclonal antibodies were obtained and characterized, their isotype and specificity were determined, and the epitope was localized using immunoenzymatic analysis, western blot, trypsinolysis, etc. The obtained monoclonal antibodies to the αC regions of the fibrin molecule can be a tool for studying the features of the functioning of the fibrin molecule in the polymerization process, as well as for use in the development of immunodiagnostics to determine the earliest forms of soluble fibrin.
Product Description
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Hoholinska Henriieta K.
Horidko Halyna I.
Klymenko Kateryna P.
Korolova Daria S.
Kostiuchenko Olena P.
Luhovska Nataliia E.
Stohnii Yevhen M.
Volodymyr O. Chernyshenko
Chernyshenko Tamara M.
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2024-10-06
