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Information × Registration Number 0224U032664, (0123U102634) , R & D reports Title Drosophila melanogaster as a platform for screening of novel antiviral compounds and studies of cellular mechanisms of defense against viruses popup.stage_title отримання і тестування екстрактів Head Ostash Bohdan O., Доктор біологічних наук Registration Date 30-11-2024 Organization Ivan Franko National University of Lviv popup.description1 Development of antiviral screening platform on the basis of special lines of insect Drosophila melanogaster expressing RNA virus Flock House Virus (FHV); investigation of this platform using model antivirals and natural extracts. popup.description2  Here it is proposed to use as a screening platform the insect, Drosophila melanogaster, expressing viral replicase FHV. We demonstrate a high toxicity of the replicase under conditions of inactivated immune control over FHV. We also show the efficiency (in terms of lethality) of neuronal expression of the replicase. Both facts attest to the feasibility of the D. melanogaster line as a screening platform. A library of natural compounds (their extracts) has been developed for the screening purposes. We assessed the content of various natural products in the extracts, their total bioactivity and heavy metal content (for plant extracts). The availability of screening platform and extract libraries constitute two necessary components of a screening system, which will be realized in the next stage of the project. Product Description popup.authors Horin Mari Ye. Makar Orysia O. Matiitsiv Nataliia P Romaniuk Natalila D. popup.nrat_date 2024-11-30 Close