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Registration Number

0224U032664, (0123U102634) , R & D reports

Title

Drosophila melanogaster as a platform for screening of novel antiviral compounds and studies of cellular mechanisms of defense against viruses

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отримання і тестування екстрактів

Head

Ostash Bohdan O., Доктор біологічних наук

Registration Date

30-11-2024

Organization

Ivan Franko National University of Lviv

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Development of antiviral screening platform on the basis of special lines of insect Drosophila melanogaster expressing RNA virus Flock House Virus (FHV); investigation of this platform using model antivirals and natural extracts.

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 Here it is proposed to use as a screening platform the insect, Drosophila melanogaster, expressing viral replicase FHV. We demonstrate a high toxicity of the replicase under conditions of inactivated immune control over FHV. We also show the efficiency (in terms of lethality) of neuronal expression of the replicase. Both facts attest to the feasibility of the D. melanogaster line as a screening platform. A library of natural compounds (their extracts) has been developed for the screening purposes. We assessed the content of various natural products in the extracts, their total bioactivity and heavy metal content (for plant extracts). The availability of screening platform and extract libraries constitute two necessary components of a screening system, which will be realized in the next stage of the project.

Product Description

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Horin Mari Ye.
Makar Orysia O.
Matiitsiv Nataliia P
Romaniuk Natalila D.

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2024-11-30