Registration Number
0224U033071,
(0121U107839)
, R & D reports
Title
Scientific bases of manifestation of regenerative ability of grapes and ways of its increase in the process of production of planting material of basic and certified categories
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Створення та обґрунтування системи експериментальних даних щодо прояву морфогенної, неморфогенної регенерації щеп, мікроклонів винограду
Head
Zelenianska Natalia М., Доктор сільськогосподарських наук
Registration Date
17-12-2024
Organization
National Research Center "VE Tairov Institute of Viticulture and Enology"
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To study the peculiarities of the regenerative-restorative processes of cuttings, microclones of grapes after injury of peripheral tissues, the influence of physical, biological, biotechnological factors and to develop effective ways to increase the regenerative capacity of grape planting material of different quality categories.
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The research report is written on 26 pages, contains 10 tables, 2 figures, 19 references.
It was found that the most favorable for the survival and proliferation of explant buds of grape varieties Arkadiya and Zagadka was a nutrient medium with antioxidants 1.5 mg/l ascorbic acid and 0.5% glycine both under etiolation conditions and under lighting with a 12-hour photoperiod. The content of polyphenolic compounds in the green tissues of experimental explants of these variants was lower than in the control, 15 days after the introduction after the end of the etiolation period, and after 30 days the amount of polyphenols increased, which, combined with good biometric indicators of microclones, indicates optimal conditions for growth and development of microclones. Callus cultures were obtained from leaf explants of grapevines of technical and rootstock varieties on a nutrient medium MS containing 3.0 mg/l NOC and 0.5 mg/l 6-BAP. The best medium for co-cultivation of calluses was the nutrient medium MS with 2.5 mg/l IOC, 1 mg/l 6-BAP. The obtained callus cultures had an average degree of development, which indicates partial tissue compatibility.
Calculations of the regeneration potential of chubuks for grafting showed that the highest this indicator was observed in two rootstock varieties Dobrynya, R*G 3562 – 18. Varieties R*R 101-14 4923, Kardishash, Odessa black – 9, the most optimal combination for grafting, taking into account the simultaneous dates of the beginning of callusogenesis and the formation of a circular callus on the sections. Rootstocks B*R СО4 1791 and P*G 5941 almost did not show regeneration properties, the sections on the surface were oxidized, which may indicate inhibition of callusogenesis due to the formation of oxidation products of polyphenolic compounds when interacting with cell enzymes and active forms of oxygen.
The analysis of the dependence between shoot humidity and R showed the most noticeable relationship (R2=0.4365; r= -0.66) which i
Product Description
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Ivashko Valentyna M.
Artiukh Mykola M.
Boiko Olena M.
Borun Vasyl V.
Hohulinska Olena I.
Samofalov Mykhailo O.
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2024-12-17
