Registration Number
0224U033144,
(0124U004386)
, R & D reports
Title
Implementation of 2D bi-layered nanomembranes for guided tissue regeneration in endo-perio lesions and periimplantitis
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Розробка технології рівномірного нанесення наночастинок срібла (AgNP) та фактору росту фібробластів FGF2-STAB на нановолокнисті мембрани, виготовлені методом електропрядіння. Встановлення впливу модифікації полімерних мембран наночастинками срібла та фактором росту FGF2-STAB на біосумісність композитних полімерних мембран з оцінкою росту клітин на модифікованих мембранах при стимуляції фактором росту FGF2-STAB
Head
Kyrylenko Serhii D., Кандидат біологічних наук
Registration Date
19-12-2024
Organization
Sumy State University
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develop 2D nanomaterials (nanomats) filled with antibacterial agents (silver nanoparticles) and growth factor (GF) which will simultaneously prevent endo- periodontal infection and promote guided tissue regeneration to maintain oral health
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Innovative antimicrobial materials based on electrospun nanofibrous membranes composed of chitosan (Ch) and polylactic acid (PLA), modified with polyethylene glycol (PEG) and silver nanoparticles (AgNPs), were developed in this study. SEM analysis revealed that the addition of PLA and PEG to the solution facilitated the formation of uniform fibers with an average diameter of 150–200 nm, with PEG-containing samples exhibiting an increased number of nanopores smaller than 50 nm². The incorporation of AgNPs (at concentrations of 50, 100, 200, and 400 µg/mL) significantly enhanced the antibacterial properties of the membranes, with samples containing 400 µg/mL achieving 95% growth inhibition of Escherichia coli and 92% of Staphylococcus aureus within the first 6 hours.
When testing antiadhesive properties in an environment with S. aureus biofilms, membranes modified with PEG demonstrated a 65% reduction in biofilm formation compared to samples without PEG. For E. coli, biofilm formation was almost negligible, indicating distinct mechanisms of action against gram-positive and gram-negative bacteria.
The addition of PEG improved the hydrophilicity of the membranes, reducing the contact angle from 120° to 50°, which also contributed to decreased bacterial adhesion. Cytotoxicity studies showed that all samples were biocompatible, with the metabolic activity of mesenchymal stem cells (MSC) on PEG-containing membranes increasing by 15% due to enhanced hydrophilicity.
The application of combined fabrication technologies to produce composite materials, such as electrospun Ch/PLA nanofibers modified with AgNPs, opens new avenues for the development of highly effective antimicrobial biomaterials. Overall, the obtained membranes exhibit significant potential for use in antibacterial coatings and medical biomaterials with improved compatibility with human tissues
Product Description
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Bulyha Iryna O.
Varava Yuliia V.
Diedkova Kateryna А.
Dryhval Bohdan О.
Kyrylenko Serhii D.
Samokhin Yevhen O.
Chorna Inna V.
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2024-12-19
